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RNA

Part:BBa_K1017404:Design

Designed by: Wen-Hui Cheng   Group: iGEM13_NCTU_Formosa   (2013-09-12)

sRNA-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the purpose of the sRNA-2 is to bind on the corresponding RBS sequence in order to block the following translation.

Source

synthesis

References

Torsten Waldminghaus, Nadja Heidrich, Sabine Brantl and Franz Narberhaus .(2012). Engineering Artificial Small RNAs for Conditional Gene Silencing in Escherichia coli ,1: 6–13